It’s insane to see the advances this has made in the Funny Biology Teachers 2020 The One Where They Were Quarantined Social Distancing T-Shirt, even. I managed a research lab from ’08-’12 that dealt with in-vivo imaging for vision development. We used cats as a model (sort of contrary to what the paper says, you don’t have to genetically engineer animals for calcium imaging) to record clusters of neurons firing in response to 3D image stimuli. Now they can do zebrafish larvae free-swimming.
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Yeah, it wasn’t the imaging itself (or dye use) that was annoying, it was everything else. Have to scalp the animal, have to do a Funny Biology Teachers 2020 The One Where They Were Quarantined Social Distancing T-Shirt, mount a stabilization plate, etc. We had to have the animals completely still. Hell, we had to use a floating table to remove vibrations from the building. Tell me about it. Did it for years (just defended in April), but I could never get the prep to take less than an hour and a half or so…and if I did ANYTHING wrong (god forbid I nick a blood vessel), my recording was essentially screwed. Mine never had to be incredibly still (didn’t use an air table or anything), but there was still a ton of hardware that was a pain. The good news is that I was able to get through a TON of podcasts during that time.
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