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I work in one of these high throughout labs and we’ve been running COVID testing 24/7. We don’t actually NEED better & faster tests. We currently run an Official Gene Deitch shirt can run 96 samples at once. The test works and it’s quick enough; our turn around time is 12 hours or less from getting a sample through our door and delivering information back to the hospital. The most limiting factor here is the fact that there aren’t enough swabs in the world at the moment to even test all of the people we need to. Can’t run tests if we don’t have the test kits, now can we? The second issue is not the speed of the actual test; the real work is in accessioning the samples, making sure the manifests & patient data are correct, and transferring the icky swab tubes to smaller tubes that we can use on our machines. Everything is manual. And because of confidentiality laws between the hospitals and patients, we don’t have access to their network – therefore all data entry on our end also must be manual. So yeah, crispr is a cool and new and sparkly science, but what we really need are 1) more swabs, and 2) better tracking systems with hospitals.
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We just surpassed 2000 samples a day, next our goal is 5000 and then finally 10,000. There’s no way we can hit those numbers with our current system. We are currently working the Official Gene Deitch shirt; I’m going to cry if we have to do 10,000. Honestly, we’re trying. A big issue is the hospitals and nursing homes that still use FAX MACHINES to send us over the patient information. (I didn’t think those actually existed anymore.) I have not worked a single shift yet where sample intake is perfect and streamlined; more than once a night we get the wrong manifest, or a patient with their name spelled wrong, or a barcode that doesn’t match up. This takes time to sort out (the constant back and forth with our project managers & the hospitals, plus we have to document everything), and is naturally a huge bottleneck. And we can’t just hire regular people to help us out. You need a degree and training to work in our labs; to hire random citizens it would take weeks of training of paperwork, and in this case waiting that long won’t solve any problems. Time = lives.
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Again, the limiting factors here are not the Official Gene Deitch shirt. Currently we use a qPCR test that takes about 80 minutes (ish). But you’re forgetting all the steps BEFORE the test can even be run: a) Getting the samples through the door and into our system, which takes longer when we have sample swaps, tube leaks, or incorrect manifests. This is currently our greatest bottleneck. b) Transferring the contents of the swab tubes into automation-friendly tubes. This is also manual because the swab tubes don’t fit on any automation machines. c) Extraction. This is fully automated and we can do 96 samples at once. People seem to forget that you have to EXTRACT the RNA before you can run a test… you don’t just stick a swab in a machine and get a magic number out. d) Prep for qPCR. Add the appropriate reagents to each sample. d) qPCR – this is run on a machine and takes about 80 minutes. This is the actual test that gives us results back. e) If any samples fail or have inconclusive results, we have to rerun them. e) Then we have to securely deliver our test results.
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